GAPDH-Monoclonal Antibodies

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GAPDH

Qty


Total
$135
Catalog #
AC001
Antibody Type
Polyclonal Antibody
Gene ID
2597
Swiss Prot
P04406
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
Reactivity Human Mouse Rat
Tested applications WB IHC IF
Recommended Dilution WB 1:1000 - 1:10000 IHC 1:50 - 1:100 IF 1:20 - 1:50
Calculated MW 36kDa
Observed MW Refer to Figures
Immunogen Recombinant protein of human GAPDH
Storage Buffer Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonym GAPDH;G3PD;GAPD;MGC88685 ;
Images
  • AC001: image 1

    Immunohistochemistry of paraffin-embeddedrat pancreas using GAPDH antibody at dilution 1:100(400x lens).

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transport, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PACR and also loading control in electrophoresis and Western blotting. This antibody is a rabbit polyclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Protocol

N/A

MSDS
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