TRIM21-Monoclonal Antibodies

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TRIM21

Qty


Total
$220
Catalog #
A1957
Antibody Type
Polyclonal Antibody
Gene ID
6737
Swiss Prot
P19474
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
Reactivity Human Mouse
Tested applications WB IHC IF
Recommended Dilution WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Calculated MW 54kDa
Observed MW Refer to Figures
Immunogen Recombinant protein of human TRIM21
Storage Buffer Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration l
Synonym RNF81; RO52; SSA; SSA1;
Images
  • A1957: image 1

    Western blot analysis of extracts of various cell lines, using TRIM21 antibody.

  • A1957: image 2

    Immunohistochemistry of paraffin-embedded mouse heart using TRIM21 antibody at dilution of 1:100 (x40 lens).

  • A1957: image 3

    Immunofluorescence analysis of U2OS cell using TRIM21 antibody. Blue: DAPI for nuclear staining.

Background

TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM3 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules.

Protocol

N/A

MSDS
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