GLC8 Yeast-Other

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GLC8 Yeast

Qty


Total
$65
Catalog #
PRPS-547
Size
Description
GLC8 Yeast (Saccharomyces cerevisiae) Recombinant produced in E.coli is a single, glycosylated polypeptide chain containing 265 amino acids (1-229) and having a molecular mass of 30.7 kDa (molecular size on SDS-PAGE will appear higher). The GLC8 is fused to a 35 amino acid His Tag at N-Terminus and purified by standard chromatography techniques.
Additional Information
Introduction GLC8 is a regulatory subunit of protein phosphatase 1 (Glc7p). GLC8 plays a role in glycogen metabolism and chromosome segregation, and regulates Glc7p activity throµgh conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2. Under regular conditions, GLC8 triggers GLC7, but when GLC8 is over epxressed, it inhibits Glc7p function. GLC8 is activated upon phosphoylation by Pho85p complexed with four cyclins (Pcl6p, Pcl7p, Pcl8p, or Pcl10p).
Synonyms Protein GLC8, GLC8, YMR311C, YM9924.03C.
Source Escherichia Coli.
Physical Appearance Sterile Filtered colorless solution.
Formulation The protein solution (1mg/ml) contains 20mM Tris-HCl pH-8, 1mM DTT, and 10% glycerol.
Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Amino Acid Sequence MRGSHHHHHH
GMASMTGGQQ
MGRDLYDDDD
KDRWGSMGGI
LKNPLALSPE
QLAQQDPETL
EEFRRQVYEN
TQKNAKLTSH
KRNIPGLDNT
KEEGEIIGTS
STFLPKDTLS
LKHEQDMLAK
MTPEERVQWN
QRNLAENEIT
KKQFQDIHID
EPKTPYQGAV
DPHGEYYRVD
DDEDEDNSDK
KPCQVANDDI
DDLSLGEPEF
EIKENKQPDF
ETNDENDEDS
PEARHKKFEE
MR
Purity Greater than 95% as determined by SDS-PAGE.
Usage NeoBiolab's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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