MAP E.coli-Peptidase

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MAP E.coli

Qty


Total
$65
Catalog #
ENPS-130
Size
Description
MAP produced in E.Coli is a single, non-glycosylated polypeptide chain containing 284 amino acids (1-264 a.a.) and having a molecular mass of 31.5kDa.MAP is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
Introduction Methionine aminopeptidases and designated peptidase M proteins belong to the M24 family of proteins. MAP protein removes the amino-terminal methionine residue from nascent polypeptides. The active site of MAP contains 2 adjacent divalent metal ions connected by a water molecule or hydroxide ion.
Synonyms Methionine aminopeptidase, MAP, Peptidase M, map, b0168, JW0163.
Source Escherichia Coli.
Physical Appearance Sterile Filtered clear solution.
Formulation MAP protein solution (1mg/ml) 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 2mM DTT.
Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid Sequence MGSSHHHHHH
SSGLVPRGSH
MAISIKTPED
IEKMRVAGRL
AAEVLEMIEP
YVKPGVSTGE
LDRICNDYIV
NEQHAVSACL
GYHGYPKSVC
ISINEVVCHG
IPDDAKLLKD
GDIVNIDVTV
IKDGFHGDTS
KMFIVGKPTI
MGERLCRITQ
ESLYLALRMV
KPGINLREIG
AAIQKFVEAE
GFSVVREYCG
HGIGRGFHEE
PQVLHYDSRE
TNVVLKPGMT
FTIEPMVNAG
KK
Purity Greater than 90% as determined by SDS-PAGE.
Usage NeoBiolab's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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