PI3Kd Human-Phosphoinositide 3-kinase

PKPS-337

migration of these cells, and to impaired allergen-IgE induced degranulation and cytokine release. In neutrophils a role for PI3K? in TNFa-induced signaling was demonstrated by a reduction in Aktphosphorylation and PDK1 activity upon treatment with the d-specific inhibitor IC87114. The catalytic subunit carries a GST-Tag which facilitates the protein’s application in typical GST pull-down assays and the heterodimer was purified by affinity chromatography. The molecular weight for p110? chain is 120.2 kDa (without Tag) and the p85? chain 83.5 kDa.